Application
A. Western blot analysis on (A) HeLa, (B) HUVEC, (C) NIH/3T3 and (D) C6 cell lysates using anti-RPA32 RabMAb (Cat. #AJ1695a), dilution 1:10,000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-RPA32 RabMAb (Cat. #AJ1695a).| Product Name | RPA32 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RPA2, REPA2, RPA32, RPA34, Replication protein A 32 kDa subunit, RP-A p34;Replication factor A protein 2 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2877Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | REPA2 RPA32 RPA34 |
|---|---|
| Alternative Names | RPA2 REPA2 RPA32 RPA34 Replication protein A 32 kDa subunit RP-A p34 Replication factor A protein 2 |
| Molecular Weight(MW) | 29247 Da |
| Function | Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage |
| Tissue Specificity | A synthetic peptide corresponding to residues on the C terminus of human RPA32 was used as an immunogen. |
| Cellular Localization | Nucleus. Nucleus, PML body. Note=Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage |
| Entrez Gene | 6118 |
|---|
Application
A. Western blot analysis on (A) HeLa, (B) HUVEC, (C) NIH/3T3 and (D) C6 cell lysates using anti-RPA32 RabMAb (Cat. #AJ1695a), dilution 1:10,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-RPA32 RabMAb (Cat. #AJ1695a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
