Application
Western blot analysis of anti-RPC5 Pab (AP1956a) in Ramos cell line lysate (35ug/lane). RPC5(arrow) was detected using the purified Pab.| Product Name | RPC5 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR3E; KIAA1452; DNA-directed RNA polymerase III subunit RPC5; DNA-directed RNA polymerase III 80 kDa polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1452 |
|---|---|
| Alternative Names | POLR3E KIAA1452 DNA-directed RNA polymerase III subunit RPC5 DNA-directed RNA polymerase III 80 kDa polypeptide |
| Molecular Weight(MW) | 79898 Da |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity) |
| Tissue Specificity | This RPC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151~181 amino acids from the N-terminal region of human RPC5. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 55718 |
|---|
Application
Western blot analysis of anti-RPC5 Pab (AP1956a) in Ramos cell line lysate (35ug/lane). RPC5(arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
