Application
Western blot analysis of RPC8 Antibody (N-term) in mouse testis tissue lysates (35ug/lane). RPC8 (arrow) was detected using the purified Pab.Application
RPC8 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RPC8 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR3H; KIAA1665; RPC8; DNA-directed RNA polymerase III subunit RPC8; DNA-directed RNA polymerase III subunit H; RNA polymerase III subunit 22.9 kDa subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1665 RPC8 |
|---|---|
| Alternative Names | POLR3H KIAA1665 RPC8 DNA-directed RNA polymerase III subunit RPC8 DNA-directed RNA polymerase III subunit H RNA polymerase III subunit 22.9 kDa subunit |
| Molecular Weight(MW) | 22918 Da |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity) |
| Tissue Specificity | This RPC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 10-40 from the N-terminal region of human RPC8. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 171568 |
|---|
Application
Western blot analysis of RPC8 Antibody (N-term) in mouse testis tissue lysates (35ug/lane). RPC8 (arrow) was detected using the purified Pab.
Application
RPC8 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
