Application
Western blot analysis of RPL13A Antibody (C-term) in K562 cell line lysates (35ug/lane). RPL13A (arrow) was detected using the purified Pab.Application
RPL13A Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPL13A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
RPL13A Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RPL13A Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RPL13A; 60S ribosomal protein L13a; 23 kDa highly basic protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | RPL13A 60S ribosomal protein L13a 23 kDa highly basic protein |
|---|---|
| Molecular Weight(MW) | 23577 Da |
| Function | Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex. Involved in methylation of rRNA |
| Tissue Specificity | This RPL13A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 170-200 from the C-terminal region of human RPL13A. |
| Cellular Localization | Cytoplasm (Probable). |
| Entrez Gene | 23521 |
|---|
Application
Western blot analysis of RPL13A Antibody (C-term) in K562 cell line lysates (35ug/lane). RPL13A (arrow) was detected using the purified Pab.
Application
RPL13A Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPL13A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
RPL13A Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
