Application
RPS6KA4 Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the RPS6KA4 antibody detected the RPS6KA4 protein (arrow).| Product Name | RPS6KA4 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RPS6KA4;MSK2; Ribosomal protein S6 kinase alpha-4; Ribosomal protein S6 kinase alpha-4; 90 kDa ribosomal protein S6 kinase 4; Ribosomal protein S6 kinase alpha-4; Nuclear mitogen- and stress-activated protein kinase 2; Ribosomal protein S6 kinase alpha-4; |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MSK2 |
|---|---|
| Alternative Names | RPS6KA4 MSK2 Ribosomal protein S6 kinase alpha-4 Ribosomal protein S6 kinase alpha-4 90 kDa ribosomal protein S6 kinase 4 Ribosomal protein S6 kinase alpha-4 Nuclear mitogen- and stress-activated protein kinase 2 Ribosomal protein S6 kinase alpha-4 |
| Molecular Weight(MW) | 85606 Da |
| Function | Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3 Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide- stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors |
| Tissue Specificity | This RPS6KA4 Antibody Antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 481-508 amino acids from the Central region of human RPS6KA4. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 8986 |
|---|
Application
RPS6KA4 Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the RPS6KA4 antibody detected the RPS6KA4 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
