Application
The anti-MSK2 Pab is used in Western blot to detect MSK2 in placenta tissue lysate. Indirect immunofluorescence analysis showed that MSK2 is predominantly localized in the nucleus of parental (10T1/2) and oncogene-transformed (Ciras-3) mouse fibroblasts. Cells were co-stained with DAPI to visualize nucleus compartment. Data is kindly provided by B. Drobic and Dr. J. Davie from University of Manitoba (Winnipeg, Canada).| Product Name | RSKB (MSK2) Antibody (C-term R321) |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | RPS6KA4; MSK2; Ribosomal protein S6 kinase alpha-4; 90 kDa ribosomal protein S6 kinase 4; Nuclear mitogen- and stress-activated protein kinase 2; Ribosomal protein kinase B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MSK2 |
|---|---|
| Alternative Names | RPS6KA4 MSK2 Ribosomal protein S6 kinase alpha-4 90 kDa ribosomal protein S6 kinase 4 Nuclear mitogen- and stress-activated protein kinase 2 Ribosomal protein kinase B |
| Molecular Weight(MW) | 85606 Da |
| Function | Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3 Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide- stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors |
| Tissue Specificity | This RSKB (MSK2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315~344 amino acids from the C-terminal region of human MSK2. |
| Cellular Localization | Nucleus. |
Application
The anti-MSK2 Pab is used in Western blot to detect MSK2 in placenta tissue lysate. Indirect immunofluorescence analysis showed that MSK2 is predominantly localized in the nucleus of parental (10T1/2) and oncogene-transformed (Ciras-3) mouse fibroblasts. Cells were co-stained with DAPI to visualize nucleus compartment. Data is kindly provided by B. Drobic and Dr. J. Davie from University of Manitoba (Winnipeg, Canada).| Application Notes | WB:1:100~500 |
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| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
