Application
A. Western blot analysis on Hela cell lysate using anti-RSK4 Phospho (pS235) RabMAb (Cat. #AJ1700b), dilution 1:10,000. Cells were either (A) untreated or (B) treated with Calyculin A.Application
B. Immunohistochemical analysis of paraffin-embedded human muscle tissue using anti-RSK4 Phospho (pS235) RabMAb, (Cat. #AJ1700b).| Product Name | RSK4 Antibody Phospho (pS235) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RPS6KA6, RSK4, Ribosomal protein S6 kinase alpha-6, pp90RSK4 |
| Modification | p-S235 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1524Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RSK4 |
|---|---|
| Alternative Names | RPS6KA6 RSK4 Ribosomal protein S6 kinase alpha-6 pp90RSK4 |
| Molecular Weight(MW) | 83872 Da |
| Function | Constitutively active serine/threonine-protein kinase that exhibits growth-factor-independent kinase activity and that may participate in p53/TP53-dependent cell growth arrest signaling and play an inhibitory role during embryogenesis |
| Tissue Specificity | A synthetic peptide corresponding to residues surrounding serine 235 of human Rsk 4 was used as an immunogen. |
| Cellular Localization | Cytoplasm, cytosol. Nucleus. Note=Predominantly cytosolic |
| Entrez Gene | 27330 |
|---|
Application
A. Western blot analysis on Hela cell lysate using anti-RSK4 Phospho (pS235) RabMAb (Cat. #AJ1700b), dilution 1:10,000. Cells were either (A) untreated or (B) treated with Calyculin A.
Application
B. Immunohistochemical analysis of paraffin-embedded human muscle tissue using anti-RSK4 Phospho (pS235) RabMAb, (Cat. #AJ1700b).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
