Application
RSPO4 Antibody (C-term) western blot analysis in Jurkat cell line lysates (15ug/lane).This demonstrates the RSPO4 antibody detected the RSPO4 protein (arrow).Application
RSPO4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RSPO4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
RSPO4 Antibody (C-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RSPO4 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RSPO4; C20orf182; R-spondin-4; Roof plate-specific spondin-4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C20orf182 |
|---|---|
| Alternative Names | RSPO4 C20orf182 R-spondin-4 Roof plate-specific spondin-4 |
| Molecular Weight(MW) | 26171 Da |
| Function | Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway (By similarity) |
| Tissue Specificity | This RSPO4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the C-terminal region of human RSPO4. |
| Cellular Localization | Secreted (By similarity). |
| Entrez Gene | 343637 |
|---|---|
| Nucleotide Accession | NP_001025042.2 |
Application
RSPO4 Antibody (C-term) western blot analysis in Jurkat cell line lysates (15ug/lane).This demonstrates the RSPO4 antibody detected the RSPO4 protein (arrow).
Application
RSPO4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RSPO4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
RSPO4 Antibody (C-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
