Application
Western blot analysis of RUVBL1 Antibody in MCF-7 cell line lysates (35ug/lane). RUVBL1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with RUVBL1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
RUVBL1 Antibody flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of RUVBL1 (arrow) using rabbit polyclonal RUVBL1 Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RUVBL1 gene.| Product Name | RUVBL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RUVBL1; INO80H; NMP238; TIP49; TIP49A; RuvB-like 1; 49 kDa TATA box-binding protein-interacting protein; 54 kDa erythrocyte cytosolic protein; INO80 complex subunit H; Nuclear matrix protein 238; Pontin 52; TIP49a; TIP60-associated protein 54-alpha |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | INO80H NMP238 TIP49 TIP49A |
|---|---|
| Alternative Names | RUVBL1 INO80H NMP238 TIP49 TIP49A RuvB-like 1 49 kDa TATA box-binding protein-interacting protein 54 kDa erythrocyte cytosolic protein INO80 complex subunit H Nuclear matrix protein 238 Pontin 52 TIP49a TIP60-associated protein 54-alpha |
| Molecular Weight(MW) | 50228 Da |
| Function | Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair May be able to bind plasminogen at cell surface and enhance plasminogen activation |
| Tissue Specificity | Ubiquitously expressed with high expression in heart, skeletal muscle and testis |
| Cellular Localization | Nucleus matrix. Nucleus, nucleoplasm. Cytoplasm. Membrane. Cytoplasm, cytoskeleton, centrosome Note=Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells |
| Entrez Gene | 8607 |
|---|
Application
Western blot analysis of RUVBL1 Antibody in MCF-7 cell line lysates (35ug/lane). RUVBL1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with RUVBL1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
RUVBL1 Antibody flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of RUVBL1 (arrow) using rabbit polyclonal RUVBL1 Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RUVBL1 gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
