Application
RYK (Cat. #AM1912b) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the RYK antibody detected the RYK protein (arrow).| Product Name | RYK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RYK; JTK5A; Tyrosine-protein kinase RYK |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 240CT2.2.4 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | JTK5A |
|---|---|
| Alternative Names | RYK JTK5A Tyrosine-protein kinase RYK |
| Molecular Weight(MW) | 67507 Da |
| Function | May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C- terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development |
| Tissue Specificity | Observed in all the tissues examined. |
| Cellular Localization | Membrane; Single-pass type I membrane protein (By similarity). Nucleus (By similarity). Cytoplasm (By similarity). Note=In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus (By similarity) |
| Entrez Gene | 6259 |
|---|---|
| Nucleotide Accession | NP_001005861.1 NP_002949.2 |
Application
RYK (Cat. #AM1912b) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the RYK antibody detected the RYK protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
