Application
Western blot analysis of S100B Antibody in mouse liver tissue lysates (35ug/lane).S100B (arrow) was detected using the purified Pab.Application
S100B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human malignant melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of S100B Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
S100B Antibody flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | S100B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | S100B; Protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | S100B Protein S100-B S-100 protein beta chain S-100 protein subunit beta S100 calcium-binding protein B |
|---|---|
| Molecular Weight(MW) | 10713 Da |
| Function | Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization |
| Tissue Specificity | Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 6285 |
|---|
Application
Western blot analysis of S100B Antibody in mouse liver tissue lysates (35ug/lane).S100B (arrow) was detected using the purified Pab.
Application
S100B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human malignant melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of S100B Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
S100B Antibody flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by protein affinity purification. |
