Application
S4A5 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the S4A5 antibody detected the S4A5 protein (arrow).Application
S4A5 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the S4A5 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | S4A5 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SLC4A5; NBC4; Electrogenic sodium bicarbonate cotransporter 4; NBCe2; Solute carrier family 4 member 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NBC4 |
|---|---|
| Alternative Names | SLC4A5 NBC4 Electrogenic sodium bicarbonate cotransporter 4 NBCe2 Solute carrier family 4 member 5 |
| Molecular Weight(MW) | 126255 Da |
| Function | Mediates sodium- and bicarbonate-dependent electrogenic sodium bicarbonate cotransport, with a Na(+):HCO3(-) stoichiometry of 2:1. May have a housekeeping function in regulating the pH of tissues in which it is expressed. May play a role in mediating Na(+):HCO3(-) cotransport in hepatocytes and intrahepatic cholangiocytes. Also may be important in protecting the renal paranchyma from alterations in urine pH |
| Tissue Specificity | Highest expression observed in liver, spleen and testis; moderate expression in the choroid plexus, hippocampus, cerebrum and cerebellum of brain, and in kidney cortex and kidney medulla. Also observed in heart, pancreas, muscle, lung, placenta, stomach and small intestine. Weakest expression seen in peripheral blood lymphocytes, colon, duodenum, jejunum, ileum and skeletal muscle |
| Cellular Localization | Apical cell membrane; Multi-pass membrane protein. Note=Expressed in the apical plasma membrane domain of a subset of collecting ducts in the renal medulla |
| Entrez Gene | 57835 |
|---|---|
| Nucleotide Accession | NP_067019.3 NP_597812.1 |
Application
S4A5 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the S4A5 antibody detected the S4A5 protein (arrow).
Application
S4A5 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the S4A5 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
