Application
SAMSN1 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SAMSN1 antibody detected the SAMSN1 protein (arrow).Application
SAMSN1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SAMSN1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SAMSN1 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SAMSN1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SAMSN1; HACS1; SAM domain-containing protein SAMSN-1; Hematopoietic adaptor containing SH3 and SAM domains 1; Nash1; SAM domain, SH3 domain and nuclear localization signals protein 1; SH3-SAM adaptor protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HACS1 |
|---|---|
| Alternative Names | SAMSN1 HACS1 SAM domain-containing protein SAMSN-1 Hematopoietic adaptor containing SH3 and SAM domains 1 Nash1 SAM domain SH3 domain and nuclear localization signals protein 1 SH3-SAM adaptor protein |
| Molecular Weight(MW) | 41708 Da |
| Function | Negative regulator of B-cell activation. Down-regulates cell proliferation (in vitro). Promotes RAC1-dependent membrane ruffle formation and reorganization of the actin cytoskeleton Regulates cell spreading and cell polarization. Stimulates HDAC1 activity. Regulates LYN activity by modulating its tyrosine phosphorylation (By similarity) |
| Tissue Specificity | Detected in peripheral blood B-cells (at protein level). Detected in spleen, liver and peripheral blood |
| Cellular Localization | Nucleus. Cytoplasm (By similarity). Cell projection, ruffle (By similarity). Note=Shuttles between cytoplasm and nucleus. Colocalizes with the actin cytoskeleton and actin-rich membrane ruffles (By similarity) |
| Entrez Gene | 64092 |
|---|---|
| Nucleotide Accession | NP_071419.3 |
Application
SAMSN1 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SAMSN1 antibody detected the SAMSN1 protein (arrow).
Application
SAMSN1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SAMSN1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SAMSN1 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
