Application
Western blot analysis of SAP1 antibody (C-term) (Cat.# AP8429a) in K562 cell line lysates (35ug/lane). SAP1 (arrow) was detected using the purified Pab.| Product Name | SAP1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTPRH; SAP1; Receptor-type tyrosine-protein phosphatase H; Stomach cancer-associated protein tyrosine phosphatase 1; Transmembrane-type protein-tyrosine phosphatase type H |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SAP1 |
|---|---|
| Alternative Names | PTPRH SAP1 Receptor-type tyrosine-protein phosphatase H Stomach cancer-associated protein tyrosine phosphatase 1 Transmembrane-type protein-tyrosine phosphatase type H |
| Molecular Weight(MW) | 122352 Da |
| Function | May contribute to contact inhibition of cell growth and motility by mediating the dephosphorylation of focal adhesion- associated substrates and thus negatively regulating integrin- promoted signaling processes. Induces apoptotic cell death by at least two distinct mechanisms: inhibition of cell survival signaling mediated by PI 3-kinase, Akt, and ILK and activation of a caspase-dependent proapoptotic pathway. Inhibits the basal activity of LCK and its activation in response to TCR stimulation and TCR-induced activation of MAP kinase and surface expression of CD69. Inhibits TCR-induced tyrosine phosphorylation of LAT and ZAP70. Inhibits both basal activity of DOK1 and its CD2-induced tyrosine phosphorylation. Induces dephosphorylation of p130cas, focal adhesion kinase and c-Src. Reduces migratory activity of Jurkat cells |
| Tissue Specificity | Expressed at high levels in the brain, spleen and liver and at lower levels in the heart and stomach. Expressed in pancreatic and colorectal cancer cells, but not in normal pancreas or colon. Expression in hepatocellular carcinoma is related to the differentiation status of the tumor and expression is inversely related to tumor aggressiveness |
| Cellular Localization | Membrane; Single-pass type I membrane protein (Potential). Cytoplasm |
Application
Western blot analysis of SAP1 antibody (C-term) (Cat.# AP8429a) in K562 cell line lysates (35ug/lane). SAP1 (arrow) was detected using the purified Pab.| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
