Application
ELK4 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ELK4 antibody detected the ELK4 protein (arrow).Application
ELK4 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ELK4 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ELK4; SAP1; ETS domain-containing protein Elk-4; Serum response factor accessory protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SAP1 |
|---|---|
| Alternative Names | ELK4 SAP1 ETS domain-containing protein Elk-4 Serum response factor accessory protein 1 |
| Molecular Weight(MW) | 46900 Da |
| Function | Involved in both transcriptional activation and repression. Interaction with SIRT7 leads to recruitment and stabilization of SIRT7 at promoters, followed by deacetylation of histone H3 at 'Lys-18' (H3K18Ac) and subsequent transcription repression. Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously |
| Tissue Specificity | This ELK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-187 amino acids from the Central region of human ELK4. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 2005 |
|---|---|
| Nucleotide Accession | NP_001964.2 |
Application
ELK4 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ELK4 antibody detected the ELK4 protein (arrow).
Application
ELK4 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
