Application
Western blot analysis of SAMD8 Antibody (N-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse liver tissue lysate. SAMD8 Antibody (N-term) (arrow) was detected using the purified Pab (1:60/250 dilution).| Product Name | SAMD8 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SAMD8; Sphingomyelin synthase-related protein 1; Sterile alpha motif domain-containing protein 8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SAMD8 Sphingomyelin synthase-related protein 1 Sterile alpha motif domain-containing protein 8 |
|---|---|
| Molecular Weight(MW) | 48321 Da |
| Function | Sphingomyelin synthases synthesize sphingolipids through transfer of a phosphatidyl head group on to the primary hydroxyl of ceramide. SAMD8 is an endoplasmic reticulum (ER) transferase that has no sphingomyelin synthase activity but can convert phosphatidylethanolamine (PE) and ceramide to ceramide phosphoethanolamine (CPE) albeit with low product yield. Appears to operate as a ceramide sensor to control ceramide homeostasis in the endoplasmic reticulum rather than a converter of ceramides Seems to be critical for the integrity of the early secretory pathway |
| Tissue Specificity | This SAMD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-75 amino acids from the N-terminal region of human SAMD8. |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein |
| Entrez Gene | 142891 |
|---|---|
| Nucleotide Accession | NP_653261.1 |
Application
Western blot analysis of SAMD8 Antibody (N-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse liver tissue lysate. SAMD8 Antibody (N-term) (arrow) was detected using the purified Pab (1:60/250 dilution).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
