Application
Western blot analysis of SAR1A Antibody (Center) in 293(lane 1) and mouse lung tissue(lane 2) lysates (35ug/lane). SAR1A (arrow) was detected using the purified Pab.Application
Western blot analysis of SAR1A (arrow) using rabbit polyclonal SAR1A Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SAR1A gene.Application
Formalin-fixed and paraffin-embedded human lung carcinoma with SAR1A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of NCI-H292 cells using SAR1A Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SAR1A Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SAR1A; SAR1; SARA; SARA1; GTP-binding protein SAR1a; COPII-associated small GTPase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SAR1 SARA SARA1 |
|---|---|
| Alternative Names | SAR1A SAR1 SARA SARA1 GTP-binding protein SAR1a COPII-associated small GTPase |
| Molecular Weight(MW) | 22367 Da |
| Function | Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES) |
| Tissue Specificity | This SAR1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128~158 amino acids from the Center region of human SAR1A. |
| Cellular Localization | Endoplasmic reticulum (By similarity). Golgi apparatus (By similarity) |
| Entrez Gene | 56681 |
|---|
Application
Western blot analysis of SAR1A Antibody (Center) in 293(lane 1) and mouse lung tissue(lane 2) lysates (35ug/lane). SAR1A (arrow) was detected using the purified Pab.
Application
Western blot analysis of SAR1A (arrow) using rabbit polyclonal SAR1A Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SAR1A gene.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma with SAR1A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of NCI-H292 cells using SAR1A Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
