Application
The anti-SARS-Sn Pab is used in Western blot to detect recombinant Spike proteins, aa17-537 (Lane 1) and aa17-756 (Lane 2).| Product Name | SARS virus Sn Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | S; Spike glycoprotein; E2; Peplomer protein; Spike protein S1; Spike protein S2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | S Spike glycoprotein E2 Peplomer protein Spike protein S1 Spike protein S2 |
|---|---|
| Molecular Weight(MW) | 139125 Da |
| Function | S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes |
| Tissue Specificity | This SARS virus Sn antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13~42 amino acids from the N-terminus of SARS CoV Spike protein. |
| Cellular Localization | Virion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein (By similarity) Host cell membrane; Single-pass type I membrane protein Note=Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly (By similarity). Some S oligomers are transported to the plasma membrane, where they may mediate cell-cell fusion |
Application
The anti-SARS-Sn Pab is used in Western blot to detect recombinant Spike proteins, aa17-537 (Lane 1) and aa17-756 (Lane 2).| Application Notes | WB:1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
