Application
Western blot analysis on (A) Jurkat, (B) Mouse Thymus lysates using anti-SATB1 RabMAb (Cat. #AJ1705a).Application
Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue using anti-SATB1 RabMAb (Cat. #AJ1705a).Application
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SATB1 RabMAb (Cat. #AJ1705a).| Product Name | SATB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3895 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Molecular Weight(MW) | 85957 Da |
|---|---|
| Function | Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1- binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly up-regulating metastasis-associated genes while down-regulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis |
| Tissue Specificity | Expressed predominantly in thymus. |
| Cellular Localization | Nucleus matrix. Nucleus, PML body. Note=Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies (PML NBs) |
| Entrez Gene | 6304 |
|---|
Application
Western blot analysis on (A) Jurkat, (B) Mouse Thymus lysates using anti-SATB1 RabMAb (Cat. #AJ1705a).
Application
Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue using anti-SATB1 RabMAb (Cat. #AJ1705a).
Application
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SATB1 RabMAb (Cat. #AJ1705a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
