Application
SCAP Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SCAP antibody detected the SCAP protein (arrow).Application
SCAP Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCAP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SCAP Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SCAP Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCAP; KIAA0199; Sterol regulatory element-binding protein cleavage-activating protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0199 |
|---|---|
| Alternative Names | SCAP KIAA0199 Sterol regulatory element-binding protein cleavage-activating protein |
| Molecular Weight(MW) | 139729 Da |
| Function | Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity) |
| Tissue Specificity | This SCAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-83 amino acids from the N-terminal region of human SCAP. |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Golgi apparatus membrane; Multi- pass membrane protein (By similarity). Cytoplasmic vesicle, COPII- coated vesicle membrane; Multi-pass membrane protein (By similarity). Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols (By similarity) |
| Entrez Gene | 22937 |
|---|---|
| Nucleotide Accession | NP_036367.2 |
Application
SCAP Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SCAP antibody detected the SCAP protein (arrow).
Application
SCAP Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCAP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SCAP Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
