Application
SCEL Antibody (Center) western blot analysis in NCI-H292,A549 cell line lysates (35ug/lane).This demonstrates the SCEL antibody detected the SCEL protein (arrow).Application
SCEL Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SCEL Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCEL; Sciellin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SCEL Sciellin |
|---|---|
| Molecular Weight(MW) | 77552 Da |
| Function | May function in the assembly or regulation of proteins in the cornified envelope. The LIM domain may be involved in homotypic or heterotypic associations and may function to localize sciellin to the cornified envelope |
| Tissue Specificity | Highly expressed in esophagus. It is also expressed in keratinocytes, amniotic tissue, foreskin stratum spinosum and stratum granulosum, hair follicle and nail |
| Cellular Localization | Cytoplasm. Membrane. Note=May become cross- linked to membrane proteins by transglutaminase |
| Entrez Gene | 8796 |
|---|---|
| Nucleotide Accession | NP_659001.2 NP_003834.3 |
Application
SCEL Antibody (Center) western blot analysis in NCI-H292,A549 cell line lysates (35ug/lane).This demonstrates the SCEL antibody detected the SCEL protein (arrow).
Application
SCEL Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
