Application
Western blot analysis of KITLG Antibody (C-term) in 293 cell line lysates (35ug/lane). KITLG (arrow) was detected using the purified Pab (1:60 dilution).Application
Western blot analysis of KITLG(arrow) using rabbit polyclonal KITLG Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KITLG gene (Lane 2) (Origene Technologies).Application
Immunofluorescence analysis of anti-KITLG Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.Application
Flow cytometric analysis of WiDr cells using SCF (KITLG) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SCF (KITLG) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KITLG; MGF; SCF; Kit ligand; Mast cell growth factor; Stem cell factor; c-Kit ligand; Soluble KIT ligand |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFFC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MGF SCF |
|---|---|
| Alternative Names | KITLG MGF SCF Kit ligand Mast cell growth factor Stem cell factor c-Kit ligand Soluble KIT ligand |
| Molecular Weight(MW) | 30899 Da |
| Function | Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins |
| Tissue Specificity | This SCF (KITLG) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244~273 amino acids from the C-terminal region of human KITLG. |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein Soluble KIT ligand: Secreted. |
| Entrez Gene | 4254 |
|---|
Application
Western blot analysis of KITLG Antibody (C-term) in 293 cell line lysates (35ug/lane). KITLG (arrow) was detected using the purified Pab (1:60 dilution).
Application
Western blot analysis of KITLG(arrow) using rabbit polyclonal KITLG Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KITLG gene (Lane 2) (Origene Technologies).
Application
Immunofluorescence analysis of anti-KITLG Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Application
Flow cytometric analysis of WiDr cells using SCF (KITLG) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
