Application
A. Western blot analysis on 5ng of recombinant protein using SCF RabMAb (Cat. #AJ1708a), dilution 1:10,000.Application
B. Immunohistochemical analysis of paraffin-embedded human kidney carcinoma using anti-SCF RabMAb (Cat. #AJ1708a).| Product Name | SCF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KITLG, MGF, SCF, Kit ligand, Mast cell growth factor |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP665Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50000~200000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MGF SCF |
|---|---|
| Alternative Names | KITLG MGF SCF Kit ligand Mast cell growth factor |
| Molecular Weight(MW) | 30899 Da |
| Function | Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins |
| Tissue Specificity | A synthetic peptide corresponding to residues near the N-terminus of human SCF was used as an immunogen. |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein Soluble KIT ligand: Secreted. |
| Entrez Gene | 4254 |
|---|
Application
A. Western blot analysis on 5ng of recombinant protein using SCF RabMAb (Cat. #AJ1708a), dilution 1:10,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human kidney carcinoma using anti-SCF RabMAb (Cat. #AJ1708a).| Application Notes | WB:1:50000~200000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
