Application
Western blot analysis of SCIN Antibody (N-term) in HL-60 cell line lysates (35ug/lane). SCIN (arrow) was detected using the purified Pab.Application
SCIN Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SCIN Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SCIN Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SCIN Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCIN; KIAA1905; Adseverin; Scinderin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1905 |
|---|---|
| Alternative Names | SCIN KIAA1905 Adseverin Scinderin |
| Molecular Weight(MW) | 80489 Da |
| Function | Ca(2+)-dependent actin filament-severing protein that is presumed to have a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane. In vitro, also has barbed end capping and nucleating activities in the presence of Ca(2+). Regulates chondrocyte proliferation and differentiation. MAP kinases p38 and ERK1/2 mediate the adseverin-induced accelerated differentiation of non-hypertrophic chondrocytes (By similarity) |
| Tissue Specificity | This SCIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human SCIN. |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Entrez Gene | 85477 |
|---|
Application
Western blot analysis of SCIN Antibody (N-term) in HL-60 cell line lysates (35ug/lane). SCIN (arrow) was detected using the purified Pab.
Application
SCIN Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SCIN Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SCIN Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
