Application
SCN1B Antibody (N-Term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the SCN1B antibody detected the SCN1B protein (arrow).Application
SCN1B Antibody (N-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SCN1B Antibody (N-Term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SCN1B Antibody (N-Term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SCN1B Antibody (N-Term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCN1B; Sodium channel subunit beta-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SCN1B Sodium channel subunit beta-1 |
|---|---|
| Molecular Weight(MW) | 24707 Da |
| Function | Crucial in the assembly, expression, and functional modulation of the heterotrimeric complex of the sodium channel The subunit beta-1 can modulate multiple alpha subunit isoforms from brain, skeletal muscle, and heart. Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons |
| Tissue Specificity | The overall expression of isoforms 1 and 2 is very similar. Isoform 1 is abundantly expressed in skeletal muscle, heart and brain. Isoform 2 is highly expressed in brain and skeletal muscle and present at a very low level in heart, placenta, lung, liver, kidney and pancreas. In brain, isoform 2 is most abundant in the cerebellum, followed by the cerebral cortex and occipital lobe, while isoform 1 levels are higher in the cortex compared to the cerebellum. Isoform 2 is expressed in many regions of the brain, including cerebellar Purkinje cells, cortex pyramidal neurons and many of the neuronal fibers throughout the brain (at protein level). Also detected in dorsal root ganglion, in fibers of the spinal nerve and in cortical neurons and their processes (at protein level) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 6324 |
|---|---|
| Nucleotide Accession | NP_950238.1 |
Application
SCN1B Antibody (N-Term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the SCN1B antibody detected the SCN1B protein (arrow).
Application
SCN1B Antibody (N-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SCN1B Antibody (N-Term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SCN1B Antibody (N-Term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
