Application
Western blot analysis of SCNN1A Antibody (Center) in WiDr cell line lysates (35ug/lane). SCNN1A (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with SCNN1A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of SCNN1A Antibody (Center) with Hela cell followed by Alexa Fluor搴?489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
SCNN1A Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SCNN1A Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCNN1A; SCNN1; Amiloride-sensitive sodium channel subunit alpha; Alpha-NaCH; Epithelial Na(+) channel subunit alpha; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SCNN1 |
|---|---|
| Alternative Names | SCNN1A SCNN1 Amiloride-sensitive sodium channel subunit alpha Alpha-NaCH Epithelial Na(+) channel subunit alpha Nonvoltage-gated sodium channel 1 subunit alpha SCNEA |
| Molecular Weight(MW) | 75704 Da |
| Function | Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands Also plays a role in taste perception |
| Tissue Specificity | Highly expressed in kidney and lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta. Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart |
| Cellular Localization | Apical cell membrane; Multi-pass membrane protein. Note=Apical membrane of epithelial cells |
| Entrez Gene | 6337 |
|---|
Application
Western blot analysis of SCNN1A Antibody (Center) in WiDr cell line lysates (35ug/lane). SCNN1A (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with SCNN1A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of SCNN1A Antibody (Center) with Hela cell followed by Alexa Fluor搴?489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
SCNN1A Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
