Application
Western blot analysis of anti-SCYL2 Pab (AP7024b) in T47D (left) and mouse brain (right) tissue lysates (35ug/lane). SCYL2(arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with SCYL2 (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | SCYL2 (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCYL2; CVAK104; KIAA1360; SCY1-like protein 2; Coated vesicle-associated kinase of 104 kDa |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CVAK104 KIAA1360 |
|---|---|
| Alternative Names | SCYL2 CVAK104 KIAA1360 SCY1-like protein 2 Coated vesicle-associated kinase of 104 kDa |
| Molecular Weight(MW) | 103709 Da |
| Function | Component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. According to PubMed:15809293, probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L- lysine. According to PubMed:16914521, has no detectable kinase activity in vitro. May regulate clathrin-dependent trafficking between the TGN and/or the endosomal system |
| Tissue Specificity | This SCYL2 (C-term) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SCYL2. |
| Cellular Localization | Cytoplasm, perinuclear region. Cytoplasmic vesicle, clathrin-coated vesicle. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Note=According to PubMed:15809293, plasma membrane-associated in clathrin-coated vesicles. According to PubMed:16914521, colocalizes to the trans- Golgi network (TGN) and to endosomal membranes with clathrin, transferrin and plasma membrane adapter AP1 and AP3 complexes |
| Entrez Gene | 55681 |
|---|
Application
Western blot analysis of anti-SCYL2 Pab (AP7024b) in T47D (left) and mouse brain (right) tissue lysates (35ug/lane). SCYL2(arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with SCYL2 (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
