Application
A. Western blot analysis on (A) Duadi and (B) HeLa cell lysates using anti-securin RabMAb (Cat. #AJ1709a), dilution 1:20,000.Application
B. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-Securin RabMAb (Cat. #AJ1709a).| Product Name | Securin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTTG1, EAP1, PTTG, TUTR1, Securin, Esp1-associated protein |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3240 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100000~500000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EAP1 PTTG TUTR1 |
|---|---|
| Alternative Names | PTTG1 EAP1 PTTG TUTR1 Securin Esp1-associated protein |
| Molecular Weight(MW) | 22024 Da |
| Function | Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53 The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation |
| Tissue Specificity | Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 9232 |
|---|
Application
A. Western blot analysis on (A) Duadi and (B) HeLa cell lysates using anti-securin RabMAb (Cat. #AJ1709a), dilution 1:20,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-Securin RabMAb (Cat. #AJ1709a).| Application Notes | WB:1:100000~500000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
