Application
Western blot analysis of Semaphorin 3E(Sema3e) (C-term) in CEM cell line lysates (35ug/lane).Sema3e (arrow) was detected using the purified Pab.Application
Western blot analysis of Semaphorin 3E(Sema3e) (C-term) in mouse lung tissue lysates (35ug/lane). Sema3e (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Semaphorin 3E(Sema3e) (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | SEMA3E Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Sema3e; Semah; Semh; Semaphorin-3E; Semaphorin-H |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Semah Semh |
|---|---|
| Alternative Names | Sema3e Semah Semh Semaphorin-3E Semaphorin-H |
| Molecular Weight(MW) | 89543 Da |
| Function | Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down- regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Plays an important role in ensuring the specificity of synapse formation |
| Tissue Specificity | Detected in neurons in the thalamus. Detected in embryonic vasculature. Developing lungs, developing skeletal elements and ventral horns of the developing neural tube Correlates positively with tumor progression |
| Cellular Localization | Secreted. |
| Entrez Gene | 20349 |
|---|
Application
Western blot analysis of Semaphorin 3E(Sema3e) (C-term) in CEM cell line lysates (35ug/lane).Sema3e (arrow) was detected using the purified Pab.
Application
Western blot analysis of Semaphorin 3E(Sema3e) (C-term) in mouse lung tissue lysates (35ug/lane). Sema3e (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Semaphorin 3E(Sema3e) (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
