Application
Western blot analysis of SEMA3A Antibody (Center) in CEM cell line lysates (35ug/lane). SEMA3A (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SEMA3A antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | SEMA3A Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SEMA3A; SEMAD; Semaphorin-3A; Semaphorin III |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SEMAD |
|---|---|
| Alternative Names | SEMA3A SEMAD Semaphorin-3A Semaphorin III |
| Molecular Weight(MW) | 88889 Da |
| Function | Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1 (By similarity) |
| Tissue Specificity | This SEMA3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 544~573 amino acids from the Center region of human SEMA3A. |
| Cellular Localization | Secreted (By similarity). |
| Entrez Gene | 10371 |
|---|
Application
Western blot analysis of SEMA3A Antibody (Center) in CEM cell line lysates (35ug/lane). SEMA3A (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SEMA3A antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Data sheet for OM220696