Application
Western blot analysis of SEMA3B Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). SEMA3B (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with SEMA3B Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
SEMA3B Antibody (C-term) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SEMA3B Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SEMA3B; SEMA5; SEMAA; Semaphorin-3B; Sema A(V); Semaphorin-V |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SEMA5 SEMAA |
|---|---|
| Alternative Names | SEMA3B SEMA5 SEMAA Semaphorin-3B Sema A(V) Semaphorin-V |
| Molecular Weight(MW) | 83122 Da |
| Function | Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons (By similarity) |
| Tissue Specificity | Expressed abundantly but differentially in a variety of neural and nonneural tissues |
| Cellular Localization | Secreted (By similarity). Endoplasmic reticulum. Note=Accumulates in the endoplasmic reticulum |
| Entrez Gene | 7869 |
|---|
Application
Western blot analysis of SEMA3B Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). SEMA3B (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with SEMA3B Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
SEMA3B Antibody (C-term) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
