Application
Western blot analysis of SEN34 Antibody (Center) in NIH-3T3 cell line lysates (35ug/lane). SEN34 (arrow) was detected using the purified Pab.| Product Name | SEN34 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TSEN34; LENG5; SEN34; tRNA-splicing endonuclease subunit Sen34; Leukocyte receptor cluster member 5; tRNA-intron endonuclease Sen34 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | LENG5 SEN34 |
|---|---|
| Alternative Names | TSEN34 LENG5 SEN34 tRNA-splicing endonuclease subunit Sen34 Leukocyte receptor cluster member 5 tRNA-intron endonuclease Sen34 |
| Molecular Weight(MW) | 33652 Da |
| Function | Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'- end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events |
| Tissue Specificity | This SEN34 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94~123 amino acids from the Central region of human SEN34. |
| Cellular Localization | Nucleus. Nucleus, nucleolus. Note=May be transiently localized in the nucleolus |
| Entrez Gene | 79042 |
|---|
Application
Western blot analysis of SEN34 Antibody (Center) in NIH-3T3 cell line lysates (35ug/lane). SEN34 (arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
