Application
Western blot analysis of PNUTL2 antibody (N-term) in 293 cell line lysates (35ug/lane). PNUTL2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PNUTL2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
PNUTL2 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SEPT4 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SEPT4; ARTS; PNUTL2; SEP4; Septin-4; Apoptosis-related protein in the TGF-beta signaling pathway; Bradeion beta; Brain protein H5; CE5B3 beta; Cell division control-related protein 2; Cerebral protein 7; Peanut-like protein 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ARTS PNUTL2 SEP4 |
|---|---|
| Alternative Names | SEPT4 ARTS PNUTL2 SEP4 Septin-4 Apoptosis-related protein in the TGF-beta signaling pathway Bradeion beta Brain protein H5 CE5B3 beta Cell division control-related protein 2 Cerebral protein 7 Peanut-like protein 2 |
| Molecular Weight(MW) | 55098 Da |
| Function | Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion. Isoform ARTS, but not the other isoforms, is required for the induction of cell death mediated by TGF-beta and by other apoptotic stimuli |
| Tissue Specificity | Widely expressed in adult and fetal tissues with highest expression in adult brain (at protein level), heart, liver and adrenal gland and fetal heart, kidney, liver and lung Also expressed in colorectal cancers and malignant melanomas Expressed in platelets |
| Cellular Localization | Cytoplasm (By similarity). Cytoplasm, cytoskeleton (By similarity). Note=In platelets, found in areas surrounding alpha-granules |
| Entrez Gene | 5414 |
|---|
Application
Western blot analysis of PNUTL2 antibody (N-term) in 293 cell line lysates (35ug/lane). PNUTL2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PNUTL2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
PNUTL2 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
