Application
Western blot analysis of SETD8 antibody (Center) in 293 cell line lysates (35ug/lane). SETD8 (arrow) was detected using the purified Pab.Application
SETD8 Antibody (Center) (RB18964) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SETD8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SETD8 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SETD8 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SETD8; KMT5A; PRSET7; SET07; SET8; N-lysine methyltransferase SETD8; H4-K20-HMTase SETD8; Histone-lysine N-methyltransferase SETD8; Lysine N-methyltransferase 5A; PR/SET domain-containing protein 07; SET domain-containing protein 8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KMT5A PRSET7 SET07 SET8 |
|---|---|
| Alternative Names | SETD8 KMT5A PRSET7 SET07 SET8 N-lysine methyltransferase SETD8 H4-K20-HMTase SETD8 Histone-lysine N-methyltransferase SETD8 Lysine N-methyltransferase 5A PR/SET domain-containing protein 07 SET domain-containing protein 8 |
| Molecular Weight(MW) | 42890 Da |
| Function | Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes |
| Tissue Specificity | This SETD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227~257 amino acids from the Center region of human SETD8. |
| Cellular Localization | Nucleus. Chromosome. Note=Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin |
| Entrez Gene | 387893 |
|---|
Application
Western blot analysis of SETD8 antibody (Center) in 293 cell line lysates (35ug/lane). SETD8 (arrow) was detected using the purified Pab.
Application
SETD8 Antibody (Center) (RB18964) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SETD8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SETD8 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
