WB
Western blot analysis of various lysates using KMT5A/SETD8 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.ICC/IF
Immunofluorescence analysis of MCF-7 cellsusing KMT5A/SETD8 Rabbit pAb.Secondary antibody: Cy3-conjugatedGoat anti-Rabbit IgG (H+L) at 1:500dilution.| Product Name | KMT5A/SETD8 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KMT5A |
|---|---|
| Gene Synonyms | SET8 SET07 SETD8 PR-Set7 PR/SET07 |
| Gene Full Name | lysine methyltransferase 5A |
| Gene Summary | The protein encoded by this gene is a protein-lysine N-methyltransferase that can monomethylate Lys-20 of histone H4 to effect transcriptional repression of some genes. The encoded protein is required for cell proliferation and plays a role in chromatin condensation. [provided by RefSeq, May 2016] |
| Alternative Names | SET8 SET07 SETD8 PR-Set7 PR/SET07 KMT5A/SETD8 |
| Molecular Weight(MW) | 43kDa(Observed band size:43kDa) |
| Cellular Localization | Chromosome, Nucleus. |
WB
Western blot analysis of various lysates using KMT5A/SETD8 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.
ICC/IF
Immunofluorescence analysis of MCF-7 cellsusing KMT5A/SETD8 Rabbit pAb.Secondary antibody: Cy3-conjugatedGoat anti-Rabbit IgG (H+L) at 1:500dilution.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
