Application
Western blot analysis of anti-SET7 Pab in mouse brain tissue lysate (35ug/lane). SET7(arrow) was detected using the purified Pab (1:70 dilution).| Product Name | SET7 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SETD7; KIAA1717; KMT7; SET7; SET9; Histone-lysine N-methyltransferase SETD7; Histone H3-K4 methyltransferase SETD7; Lysine N-methyltransferase 7; SET domain-containing protein 7; SET7/9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1717 KMT7 SET7 SET9 |
|---|---|
| Alternative Names | SETD7 KIAA1717 KMT7 SET7 SET9 Histone-lysine N-methyltransferase SETD7 Histone H3-K4 methyltransferase SETD7 Lysine N-methyltransferase 7 SET domain-containing protein 7 SET7/9 |
| Molecular Weight(MW) | 40721 Da |
| Function | Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation |
| Tissue Specificity | Widely expressed. Expressed in pancreatic islets |
| Cellular Localization | Nucleus. Chromosome (Probable). |
| Entrez Gene | 80854 |
|---|
Application
Western blot analysis of anti-SET7 Pab in mouse brain tissue lysate (35ug/lane). SET7(arrow) was detected using the purified Pab (1:70 dilution).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
