Application
SF3B4 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SF3B4 antibody detected the SF3B4 protein (arrow).| Product Name | SF3B4 (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SF3B4; SAP49; Splicing factor 3B subunit 4; Pre-mRNA-splicing factor SF3b 49 kDa subunit; SF3b50; Spliceosome-associated protein 49 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SAP49 |
|---|---|
| Alternative Names | SF3B4 SAP49 Splicing factor 3B subunit 4 Pre-mRNA-splicing factor SF3b 49 kDa subunit SF3b50 Spliceosome-associated protein 49 |
| Molecular Weight(MW) | 44386 Da |
| Function | Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12- dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron |
| Tissue Specificity | This SF3B4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157-187 amino acids from the Central region of human SF3B4. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 10262 |
|---|---|
| Nucleotide Accession | NP_005841.1 |
Application
SF3B4 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SF3B4 antibody detected the SF3B4 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
