Application
Western blot analysis of SF3A1 antibody (N-term) in 293 cell line lysates (35ug/lane). SF3A1 (arrow) was detected using the purified Pab.| Product Name | SF3A1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SF3A1; SAP114; Splicing factor 3A subunit 1; SF3a120; Spliceosome-associated protein 114 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SAP114 |
|---|---|
| Alternative Names | SF3A1 SAP114 Splicing factor 3A subunit 1 SF3a120 Spliceosome-associated protein 114 |
| Molecular Weight(MW) | 88886 Da |
| Function | Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex |
| Tissue Specificity | Ubiquitously expressed. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 10291 |
|---|
Application
Western blot analysis of SF3A1 antibody (N-term) in 293 cell line lysates (35ug/lane). SF3A1 (arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
