Application
SF3B1 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the SF3B1 antibody detected the SF3B1 protein (arrow).Application
SF3B1 Antibody (N-term) (AP13754a)immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SF3B1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | SF3B1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SF3B1; SAP155; Splicing factor 3B subunit 1; Pre-mRNA-splicing factor SF3b 155 kDa subunit; Spliceosome-associated protein 155 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SAP155 |
|---|---|
| Alternative Names | SF3B1 SAP155 Splicing factor 3B subunit 1 Pre-mRNA-splicing factor SF3b 155 kDa subunit Spliceosome-associated protein 155 |
| Molecular Weight(MW) | 145830 Da |
| Function | Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron |
| Tissue Specificity | This SF3B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-402 amino acids from the N-terminal region of human SF3B1. |
| Cellular Localization | Nucleus speckle. Note=During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm |
| Entrez Gene | 23451 |
|---|---|
| Nucleotide Accession | NP_036565.2 |
Application
SF3B1 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the SF3B1 antibody detected the SF3B1 protein (arrow).
Application
SF3B1 Antibody (N-term) (AP13754a)immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SF3B1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
