Application
Western blot analysis of SF3B14 Antibody (C-term) in Hela cell line lysates (35ug/lane). SF3B14 (arrow) was detected using the purified Pab.Application
Western blot analysis of SF3B14 Antibody (C-term) in mouse testis tissue lysates (35ug/lane). SF3B14 (arrow) was detected using the purified Pab.Application
SF3B14 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SF3B14 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SF3B14; Pre-mRNA branch site protein p14; SF3b 14 kDa subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SF3B14 Pre-mRNA branch site protein p14 SF3b 14 kDa subunit |
|---|---|
| Molecular Weight(MW) | 14585 Da |
| Function | Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively |
| Tissue Specificity | This SF3B14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96~125 amino acids from the C-terminal region of human SF3B14. |
| Cellular Localization | Nucleus (Potential). |
| Entrez Gene | 51639 |
|---|
Application
Western blot analysis of SF3B14 Antibody (C-term) in Hela cell line lysates (35ug/lane). SF3B14 (arrow) was detected using the purified Pab.
Application
Western blot analysis of SF3B14 Antibody (C-term) in mouse testis tissue lysates (35ug/lane). SF3B14 (arrow) was detected using the purified Pab.
Application
SF3B14 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
