Application
SGEF Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SGEF antibody detected the SGEF protein (arrow).Application
SGEF Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SGEF Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | SGEF Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARHGEF26; SGEF; Rho guanine nucleotide exchange factor 26; SH3 domain-containing guanine exchange factor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SGEF |
|---|---|
| Alternative Names | ARHGEF26 SGEF Rho guanine nucleotide exchange factor 26 SH3 domain-containing guanine exchange factor |
| Molecular Weight(MW) | 97346 Da |
| Function | Activates RhoG GTPase by promoting the exchange of GDP by GTP. Required for the formation of membrane ruffles during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB, which induces cytoskeleton rearrangements and promotes bacterial entry |
| Tissue Specificity | Isoform 1 is broadly expressed, with highest levels in liver (at protein level). Certain mRNA species appear to be specifically expressed in prostate and liver |
| Cellular Localization | Cell projection, ruffle. |
| Entrez Gene | 26084 |
|---|---|
| Nucleotide Accession | NP_056410.3 |
Application
SGEF Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SGEF antibody detected the SGEF protein (arrow).
Application
SGEF Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SGEF Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
