Application
Western blot analysis of SGF29 Antibody (C-term) in MDA-MB435 cell line lysates (35ug/lane).SGF29 (arrow) was detected using the purified Pab.Application
SGF29 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SGF29 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SGF29 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SGF29 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCDC101; SGF29; SAGA-associated factor 29 homolog; Coiled-coil domain-containing protein 101 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SGF29 |
|---|---|
| Alternative Names | CCDC101 SGF29 SAGA-associated factor 29 homolog Coiled-coil domain-containing protein 101 |
| Molecular Weight(MW) | 33238 Da |
| Function | Involved in transcriptional regulation, through association with histone acetyltransferase (HAT) SAGA-type complexes like the TFTC-HAT, ATAC or STAGA complexes. Specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3). In the SAGA- type complexes, required to recruit complexes to H3K4me. May be involved in MYC-mediated oncogenic transformation |
| Tissue Specificity | This SGF29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-218 amino acids from the C-terminal region of human SGF29. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 112869 |
|---|
Application
Western blot analysis of SGF29 Antibody (C-term) in MDA-MB435 cell line lysates (35ug/lane).SGF29 (arrow) was detected using the purified Pab.
Application
SGF29 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SGF29 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SGF29 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
