Application
SGMS1 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the SGMS1 antibody detected the SGMS1 protein (arrow).Application
SGMS1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SGMS1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | SGMS1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SGMS1; MOB; SMS1; TMEM23; Phosphatidylcholine:ceramide cholinephosphotransferase 1; Medulla oblongata-derived protein; Sphingomyelin synthase 1; Transmembrane protein 23 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MOB SMS1 TMEM23 |
|---|---|
| Alternative Names | SGMS1 MOB SMS1 TMEM23 Phosphatidylcholine:ceramide cholinephosphotransferase 1 Medulla oblongata-derived protein Sphingomyelin synthase 1 Transmembrane protein 23 |
| Molecular Weight(MW) | 49208 Da |
| Function | Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Golgi apparatus SMS1 directly and specifically recognizes the choline head group on the substrate, requiring two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Major form in macrophages. Required for cell growth in certain cell types such as HeLa cells. Suppresses BAX-mediated apoptosis and also prevents cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. May protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide |
| Tissue Specificity | Brain, heart, kidney, liver, muscle and stomach |
| Cellular Localization | Golgi apparatus membrane; Multi-pass membrane protein |
| Entrez Gene | 259230 |
|---|---|
| Nucleotide Accession | NP_671512.1 |
Application
SGMS1 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the SGMS1 antibody detected the SGMS1 protein (arrow).
Application
SGMS1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SGMS1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
