WB
SGMS2 Antibody (C-term) western blot analysis in MCF-7,CEM,NCI-H460 cell line lysates (35ug/lane).This demonstrates the SGMS2 antibody detected the SGMS2 protein (arrow).WB
SGMS2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SGMS2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.FC
SGMS2 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.IHC
SGMS2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SGMS2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | SGMS2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:1000 IHC:1:100-1:500 FC:1:50 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SMS2 |
|---|---|
| Alternative Names | SGMS2 SMS2 Phosphatidylcholine:ceramide cholinephosphotransferase 2 Sphingomyelin synthase 2 |
| Molecular Weight(MW) | 42280 Da |
| Function | Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Plasma membrane SMS2 can also convert phosphatidylethanolamine (PE) to ceramide phosphatidylethanolamine (CPE). Major form in liver. Required for cell growth in certain cell types. Regulator of cell surface levels of ceramide, an important mediator of signal transduction and apoptosis. Regulation of sphingomyelin (SM) levels at the cell surface affects insulin sensitivity |
| Tissue Specificity | Brain, heart, kidney, liver, muscle and stomach. Also expressed in a number of cell lines such as carcinoma HeLa cells, hepatoma Hep-G2 cells, and colon carcinoma Caco-2 cells |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein Note=Predominantly plasma membrane, some in Golgi apparatus. Some localization in the perinuclear region where it colocalizes with a sialyltransferase |
| Entrez Gene | 166929 |
|---|
WB
SGMS2 Antibody (C-term) western blot analysis in MCF-7,CEM,NCI-H460 cell line lysates (35ug/lane).This demonstrates the SGMS2 antibody detected the SGMS2 protein (arrow).
WB
SGMS2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SGMS2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
FC
SGMS2 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IHC
SGMS2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SGMS2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:1000 IHC:1:100-1:500 FC:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
