Application
SGOL2 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SGOL2 antibody detected the SGOL2 protein (arrow).| Product Name | SGOL2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SGOL2; Shugoshin-like 2; Shugoshin-2; Tripin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SGOL2 Shugoshin-like 2 Shugoshin-2 Tripin |
|---|---|
| Molecular Weight(MW) | 144739 Da |
| Function | Cooperates with PPP2CA to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Has a crucial role in protecting REC8 at centromeres from cleavage by separase. During meiosis, protects centromeric cohesion complexes until metaphase II/anaphase II transition, preventing premature release of meiosis-specific REC8 cohesin complexes from anaphase I centromeres. Is thus essential for an accurate gametogenesis. May act by targeting PPP2CA to centromeres, thus leading to cohesin dephosphorylation (By similarity). Essential for recruiting KIF2C to the inner centromere and for correcting defective kinetochore attachments |
| Tissue Specificity | This SGOL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-419 amino acids from the Central region of human SGOL2. |
| Cellular Localization | Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore (By similarity). Note=During meiosis I, accumulates at centromeres during diplotene, and co-localizes differentially with the cohesin subunits RAD21 and REC8 at metaphase I centromeres. SGO2 and RAD21 change their relative distributions during telophase I when sister-kinetochore association is lost. During meiosis II, it shows a striking tension-dependent redistribution within centromeres throughout chromosome congression during prometaphase II, as it does during mitosis (By similarity). In Hela cells, localizes at centromeres throughout prophase until metaphase and disappears at anaphase Centromeric localization requires the presence of BUB1 and AUKRB |
| Entrez Gene | 151246 |
|---|---|
| Nucleotide Accession | NP_689737.3 |
Application
SGOL2 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SGOL2 antibody detected the SGOL2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
