Application
SHARPIN Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the SHARPIN antibody detected the SHARPIN protein (arrow).| Product Name | SHARPIN Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SHARPIN; SIPL1; Sharpin; Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SIPL1 |
|---|---|
| Alternative Names | SHARPIN SIPL1 Sharpin Shank-associated RH domain-interacting protein Shank-interacting protein-like 1 |
| Molecular Weight(MW) | 39949 Da |
| Function | Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex |
| Tissue Specificity | Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Up-regulated in various tumor tissues such as kidney, liver, ovary and pancreas tumors |
| Cellular Localization | Cytoplasm, cytosol. |
| Entrez Gene | 81858 |
|---|---|
| Nucleotide Accession | NP_112236.3 |
Application
SHARPIN Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the SHARPIN antibody detected the SHARPIN protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
