Application
Western blot analysis of anti-SHB Antibody (Center) (RB13784) in CEM cell line lysates (35ug/lane). SHB (arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SHB antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | SHB Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SHB; SH2 domain-containing adapter protein B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SHB SH2 domain-containing adapter protein B |
|---|---|
| Molecular Weight(MW) | 55042 Da |
| Function | Adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic- FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associates with membrane lipid rafts upon TCR stimulation |
| Entrez Gene | 6461 |
|---|
Application
Western blot analysis of anti-SHB Antibody (Center) (RB13784) in CEM cell line lysates (35ug/lane). SHB (arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SHB antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
