Application
Western blot analysis of SHC3 Antibody (C-term E503) in mouse kidney tissue lysates (35ug/lane). SHC3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with SHC3 Antibody (C-term E503), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
SHC3 Antibody (C-term E503) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SHC3 Antibody (C-term E503) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SHC3; NSHC; SHCC; SHC-transforming protein 3; Neuronal Shc; Protein Rai; SHC-transforming protein C; Src homology 2 domain-containing-transforming protein C3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NSHC SHCC |
|---|---|
| Alternative Names | SHC3 NSHC SHCC SHC-transforming protein 3 Neuronal Shc Protein Rai SHC-transforming protein C Src homology 2 domain-containing-transforming protein C3 |
| Molecular Weight(MW) | 64056 Da |
| Function | Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons |
| Tissue Specificity | Mainly expressed in brain. Hardly detectable in other tissues, except in pancreas. Highly expressed in the cerebral cortex, frontal and temporal lobes, occipital pole, hippocampus, caudate nucleus and amygdala. Expressed at low level in the cerebellum, medulla and spinal cord |
| Entrez Gene | 53358 |
|---|
Application
Western blot analysis of SHC3 Antibody (C-term E503) in mouse kidney tissue lysates (35ug/lane). SHC3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with SHC3 Antibody (C-term E503), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
SHC3 Antibody (C-term E503) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
