Application
SHH Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the SHH antibody detected the SHH protein (arrow).Application
SHH Antibdy (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SHH Antibdy (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | SHH Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SHH; Sonic hedgehog protein; HHG-1; Sonic hedgehog protein N-product; Sonic hedgehog protein C-product |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SHH Sonic hedgehog protein HHG-1 Sonic hedgehog protein N-product Sonic hedgehog protein C-product |
|---|---|
| Molecular Weight(MW) | 49607 Da |
| Function | Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction (By similarity) |
| Tissue Specificity | Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues |
| Cellular Localization | Sonic hedgehog protein C-product: Secreted, extracellular space (By similarity). Note=The C-terminal peptide diffuses from the cell (By similarity) |
| Entrez Gene | 6469 |
|---|---|
| Nucleotide Accession | NP_000184.1 |
Application
SHH Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the SHH antibody detected the SHH protein (arrow).
Application
SHH Antibdy (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SHH Antibdy (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
