Application
A. Western blot analysis on NIH/3T3 cell lysates using anti-Phospho-SHP-2 (pY542) RabMAb (Cat. #AJ1716c). Cells were either (A) untreated or (B) treated with PDGF.| Product Name | SHP-2 Antibody Phospho (pY542) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTPN11, PTP2C, SHPTP2, Tyrosine-protein phosphatase non-receptor type 11, PTP-1D;SH-PTP3;SH-PTP2 |
| Modification | p-Y542 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP508(2)Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PTP2C SHPTP2 |
|---|---|
| Alternative Names | PTPN11 PTP2C SHPTP2 Tyrosine-protein phosphatase non-receptor type 11 PTP-1D SH-PTP3 SH-PTP2 |
| Molecular Weight(MW) | 68436 Da |
| Function | Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity |
| Tissue Specificity | Widely expressed, with highest levels in heart, brain, and skeletal muscle |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 5781 |
|---|
Application
A. Western blot analysis on NIH/3T3 cell lysates using anti-Phospho-SHP-2 (pY542) RabMAb (Cat. #AJ1716c). Cells were either (A) untreated or (B) treated with PDGF.| Application Notes | WB:1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
